Materials: Clean, healthy bulbs 70% (v/v) Alcahol Sterile water Sodium hypochlorite solution (1% available chlorine)+ 0.03 / Tween 20 (Polysorbate 20) Containers with medium: the mediumconsists of MS salts, 3% sucrose(w/v), 100mg/L myo-inositol, 0.4 mg/L thiamine-HCL, 0.27 m Mc NAA, 0.6% (w/v) agar, and a pH of 6 before autoclaving. This medium can be used both for initiation and propagation. Bulb growth in the final step can be on the same medium, but with 6% extra sucrose Method: Remove roots. Wash bulbs thoroughly with sterilized water. Remove brown or damaged outer scales. Peel off clean and healthy-looking scales. Remove brown tips. Since regeneration is best at the basal side of the scale, take care not to damage this part. Rinse scales for about 30 s with 70% alcohol. Sterilize scales for about 30 min in sodium hypochlorite. Rinse 3 times with sterile water. Cut explants of, about 7 x 7 mm. Place explants with abaxial side on medium. Explants ...