Arum Lilies Propagation Method and Materials

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Materials:
  1. Clean, healthy bulbs
  2. 70% (v/v) Alcahol
  3. Sterile water
  4. Sodium hypochlorite solution (1% available chlorine)+ 0.03 / Tween 20 (Polysorbate 20)
  5. Containers with medium: the mediumconsists of MS salts, 3% sucrose(w/v), 100mg/L myo-inositol, 0.4 mg/L thiamine-HCL, 0.27 mMc NAA, 0.6% (w/v) agar, and a pH of 6 before autoclaving. This medium can be used both for initiation and propagation. Bulb growth in the final step can be on the same medium, but with 6% extra sucrose


Method:

  1. Remove roots.
  2. Wash bulbs thoroughly with sterilized water.
  3. Remove brown or damaged outer scales.
  4. Peel off clean and healthy-looking scales. Remove brown tips. Since regeneration is best at the basal side of the scale, take care not to damage this part.
  5. Rinse scales for about 30 s with 70% alcohol.
  6. Sterilize scales for about 30 min in sodium hypochlorite.
  7. Rinse 3 times with sterile water.
  8. Cut explants of, about 7 x 7 mm.
  9. Place explants with abaxial side on medium.
  10. Explants can be cultured at 20°-25°C. In the dark, larger bulblets are obtained than in the light.
  11. After about 10 weeks, regenerated bulblets can be used for further propagation. Individual scales of the bulblets are used as explants; large scales can be cut in two or three parts. this step can be repeated every 10 weeks.
  12. After the final propagation cycle, larger bulblets ca be obtained by sub culturing single bulblets on medium with high-(6%) sucrose concentration for a number of weeks or months. This method is used to "store" the bulblets until planting time in the spring.
  13. Before planting, bulblets receive a cold treatment of several (6-12) week at 5°C in the dark.
  14. Bulblets are excised from the explant, planted in soil, and grown in a gauze house (at 17°C, most bulblets will sprout within 2-4 weeks)


Notes :

  • Before storage the bulbs are given cold treatment after harvest, and then packed in moistened sterilized peat and stored at -1°C.
  • The optimal NAA concentration for regeneration decreases with the duration of the storage time.
  •   To obtain regeneration after a hot water treatment, bulbs should be stored dry at 30°C immediately after harvest.
  • Rooting is essential for optimal growth after planting. Without roots, leaves remain small and die a few weeks later. Rooted bulblets do not need a cold treatment to break dormancy.

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